Description
M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing. ScienCell's human M-CSF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human M-CSF specific polyclonal antibody is pre-coated onto 8 x 12 strips. The human specific detection polyclonal antibody is biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human M-CSF in the sample that is captured in the strips